tkt [2019-02-19 18:18:45]

transketolase

locus: BSU17890

pI: 4.80

mw: 72.16 kDa

protein length: 667 aa

gene length: 2004 bp

function: pentose phosphate pathway

product: transketolase

essential: no

ec: 2.2.1.1

synonyms: tktA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 1,919,861 1,921,864

The protein

Catalyzed reaction/ biological activity

• Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate (according to Swiss-Prot)

Protein family

• transketolase family (according to Swiss-Prot)

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

[SW|Cofactors]

• thiamine pyrophosphate

Structure

• [PDB|3HYL], from B. anthracis

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|000E237B00539E6850B6A50EE01BA02844B6ACBC|tkt]
  description: [Pubmed|9068642]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• BS4530 ([gene|000E237B00539E6850B6A50EE01BA02844B6ACBC|tkt]::aphA3), available in [SW|Jrg Stlke]S lab
• BKE17890 ([gene|000E237B00539E6850B6A50EE01BA02844B6ACBC|tkt]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAATCCCCTTCCTTA, downstream forward: _UP4_TAAGCTTTTGAAAGAGGATG
• BKK17890 ([gene|000E237B00539E6850B6A50EE01BA02844B6ACBC|tkt]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAATCCCCTTCCTTA, downstream forward: _UP4_TAAGCTTTTGAAAGAGGATG

Expression vectors

• pGP94 (N-terminal Strep-tag, for [SW|SPINE], expression in B. subtilis, in [SW|pGP380]), available in [SW|Jrg Stlke]S lab
• for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP820, available in [SW|Jrg Stlke]S lab, [pubmed|20389117]

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]S lab

FLAG-tag construct

• GP1406 (spc, based on [SW|pGP1331]), available in [SW|Jrg Stlke]S lab

References

Reviews

• 9924800,25267444,24929114

Original publications

• 14651647,16493705,19603213,9068642,16143417,15375635,19193632,23568212,23965678,15378759